The lichen biota of the Middle Vltava Region (Central Bohemia, Czech Republic) is quite poorly known. Historical data are very sparse, but recent collections have been published in several studies. This contribution presents floristic records of 82 species of epiphytic, epixylic and terricolous lichens in the Vltava river valley and the adjacent Sedlčany region. Red-listed, regionally rare and overlooked taxa are included. The most valuable epiphytic lichens are found in xerothermic oak forests in the Vltava valley. Old beech forests are very rare in the region and occur only at higher elevations in the southern part of the area studied. Due to strong landscape eutrophication and a relatively dry and warm climate, epiphytic communities on solitary trees, in avenues and on shrubby slopes (usually former pastures) are poor in lichen species. Arthonia atra, Caloplaca lucifuga, C. monacensis, Chaenotheca hispidula, Evernia mesomorpha, Lecidea sanguineoatra, Lecidella albida, Normandina pulchella, Pachyphiale fagicola, Parmotrema perlatum, Pyrenula nitidella and Schismatomma pericleum represent the most interesting records of epiphytic lichens. Agonimia vouauxii, Cladonia peziziformis, C. scabriuscula, C. strepsilis, Epiphloea byssina, Leptogium tenuissimum, Micarea leprosula and Pycnothellia papillaria are the most valuable records of terricolous species. Many of them occur especially on sandy substrates in sandpits and in the surroundings of weathered granodiorite outcrops. Cladonia conista is reported here for the first time from the Czech Republic.
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