Podyjí is the smallest national park in the Czech Republic and mainly preserves the rocky valley of the Dyje river with extensive natural broad-leaved forests. Almost 400 lichen species have been reported from the area to date. This contribution contains records of 225 lichens and four non-lichenized fungi, which mostly come from oak forests, siliceous and calcareous rocks. The lichen Toninia pennina and the lichenicolous fungus Lichenothelia renobalesiana are reported from the Czech Republic for the first time. Buellia arborea and Calicium notarisii have been found on their second known localities in the country, Sphinctrina anglica on the third one. Caloplaca biatorina, Cladonia parasitica, Heteroplacidium compactum, Lecania sylvestris, Lecidea promixta, Lichinella stipatula, Pertusaria leucosora, P. pseudocorallina, Phaeophyscia hirsuta, Psora decipiens, Scytinium aragonii and S. teretiusculum are other remarkable species. Podyjí National Park harbours many endangered lichens and serves as an important South-Moravian refugium for them.
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