Paludella squarrosa belongs to rare and endangered bryophyte species in the Czech Republic. The historical data on its occurrence come mainly from the Bohemian-Moravian Highlands. Two unknown localities (nature reserves Zlámanec and Zlatá louka) were found recently in the northern part of this region. This paper reports them in detail, including data on water chemistry and total species composition. Hence, Paludella squarrosa is recently known at 13 localities in the Czech Republic. In both new localities, the species grows in fens with intermediate pH (6.1 and 6.9, respectively) in the Sphagno warnstorfii-Eriophoretum latifolii association. The calcium concentration in water sample from the Zlatá louka locality (68.5 mg.l-1) is the highest known one within the localities of P. squarrosa in the Bohemian-Moravian Highlands such a high calcium concentration is caused by chalk bedrock.
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