The Blanský les Mts are a small and poorly studied mountain range in South Bohemia (Czech Republic). This contribution describes the lichen diversity in one of the protected forest reserves in the area, Ptačí stěna Nature Reserve (20.53 ha, alt. 735–875 m). It is mainly covered by natural beech and scree forests of up to 200 years old. Granulite rocks and scree are abundant in the upper part of the area, but mostly shaded by the forest. In total, 156 lichen species and three non-lichenised fungi have been recorded in the reserve. Aspicilia laevata, Dirina fallax, Lecidella anomaloides, L. asema and Verrucaria acrotella are the most remarkable records of saxicolous lichens. Bacidia rosella, Gyalecta fagicola, Micarea herbarum, Schismatomma pericleum, Varicellaria hemisphaerica and Zwackhia viridis belong to the most valuable records of epiphytic (or epixylic) lichens. Three unidentified, possibly undescribed saxicolous taxa of the genera Lecidella, Sagedia and Sarcogyne are briefly discussed. Identifications of nine species are supported by molecular ITS and/or mtSSU data.