Velká Pleš National Nature Reserve (94.8 ha; alt. 248–500 m) belongs to the most valuable localities in the Křivoklátsko Protected Landscape Area. It is covered mainly by natural beech, oak and ravine forests up to ±150 years old, but the highest biodiversity is concentrated in local xerothermic steppe grasslands with andesite outcrops and in the surrounding parkland with a high tree diversity and wild mouflon grazing. In a survey between 2021 and 2023, a total of 290 lichen species, 7 lichenicolous fungi and 12 nonlichenised fungi were recorded in the reserve. Another 21 lichen species were reported in previous floristic studies but have not been confirmed recently. Presently, Velká Pleš is the fifth most lichen species-rich locality known in the Czech Republic. The corticolous fungus Morrisographium ulmi is reported here as new to the Czech Republic. Ionaspis ceracea had hitherto only been known from historical records from the country. Dermatocarpon meiophyllizum, Everhartia lignatilis (non-lichenised species) and Verrucaria substerilis are reported from their second (recent) localities in the Czech Republic. The species Bacidia vermifera, Buellia leptocline, Cladonia parasitica, Inoderma byssaceum, Multiclavula mucida, Peltigera neocanina, Pertusaria aspergilla, P. leucosora, Phaeophyscia pusilloides, Porina byssophila, Pyrenula nitidella, Rinodina moziana and Strigula affinis represent other valuable records. Identifications of 20 specimens are supported by molecular ITS and/or mtSSU data.
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