This contribution presents records of 172 lichenised fungi observed during a number of excursions to the Drahanská vrchovina Highlands, including Moravský kras (Moravian Karst) in Moravia. We studied mostly limestone localities and rock substrates. The area is rich in several species absent or very rare elsewhere in the Czech Republic, such as Caloplaca fiumana, C. xantholyta and Lecanora rouxii. The oceanic species Gyalecta nidarosiensis is reported here for the first time in the Czech Republic. We also highlight other interesting records of rarely reported species, such as Acarospora oligospora, Bagliettoa parmigera, Catapyrenium cinereum, Dirina massiliensis, Enchylium coccophorum, Endocarpon pallidum, Heteroplacidium compactum, Involucropyrenium romeanum, Lepraria diffusa, Opegrapha rupestris, Placynthium subradiatum, Protoblastenia lilacina, Rinodina immersa and Thelenella muscorum. Identifications of four specimens are supported by molecular ITS and/or mtSSU data. One collected lichen, Bacidina aff. arnoldiana, characterised by conspicuous sessile white pycnidia, probably represents an undescribed species.
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