This contribution provides records on 224 lichenized fungi and one non-lichenized fungus observed during several excursions in the Brdy highlands and the Příbram region in central Bohemia. Uranium spoil heaps in the surroundings of the town of Příbram harbour many rare species such as lichens adapted to metal-rich substrates or early succession stages. Acarospora rugulosa, A. sinopica, Bacidina brandii, Leptogium subtile and Rhizocarpon ridescens are the best examples. Lecanora flavoleprosa, traditionally known as an arctic-alpine species, is locally common in the Příbram region on siliceous stones in screes. The specific and valuable habitats on uranium spoil heaps are currently endangered by restoration activities. The localities visited in the Brdy highlands included boulder screes, a valley of a brook and avenues. Bellemerea sanguinea, Cetraria sepincola, Cladonia amaurocraea, C. bellidiflora, C. stellaris, Rhizocarpon postumum, R. timdalii represent the most important records. Surprisingly, the pyrenocarpous lichen Porina rivalis was collected in the valley of the Klabava brook at its first locality outside of Great Britain, where the species has recently been described.