This contribution presents noteworthy records of 69 saxicolous lichens from the surroundings of the Slapy and Kamýk water reservoirs and the adjacent Sedlčany Region (Central Bohemia, Czech Republic). Local diversity hot-spots are scattered among numerous rocky formations in the Vltava river valley. The high biodiversity of the lichen biota is dependent on the diversity of microclimatic, geo- morphological, geological and vegetation factors in the valley. Granodiorite rocks and various types of volcanic rocks ranging from strongly acidic to basic types predominate in the Vltava river valley. These substrata are locally rich in calciphilous species (e.g. Collema fuscovirens, C. tenax). Numerous species that are otherwise more or less rare in the Czech Republic are quite common in the river ca- nyon: Caloplaca demissa, C. irrubescens, Endocarpon psorodeum, Flavoparmelia caperata and Leprocaulon microscopicum. Endocarpon adscendens, Peltula euploca and Caloplaca viridirufa represent other widely distributed species. Acidic volcanic rocks enriched by metals, especially iron, harbour several less common lichens like Acarospora sinopica, Lecanora epanora, Lecidea silacea and Rhizo- carpon sorediosum. Rocky outcrops are rare out of the valley and are usually represented by large granitoid boulders, where Ramalina capitata is an example of a more or less common species. Three species are reported here for the first time from the Czech Republic: Endocarpon adsurgens, Pterygiopsis neglecta, and Xanthoparmelia delisei. Several other recorded lichens are rare in the Czech Republic: Acarospora irregularis, A. oligospora, Catillaria ato- marioides, Caloplaca rubelliana, Dirina stenhammari, Lecidea sarcogynoides, Leptogium magnussonii, Lichinella nigritella, Physcia tribacia, Verrucaria fuscovelutina and Xanthoparmelia angustiphylla.